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. 2009 Jan 20;106(3):797–802. doi: 10.1073/pnas.0812096106

Fig. 3.

Fig. 3.

TAp73 binding modifies BubR1 activity. TAp73 depletion decreases BubR1 activity. TAp73+/+ and TAp73−/− MEFs (A) or HeLa cells treated for 48 h with TAp73 siRNA (B) were treated with nocodazole, and extracts were immunoprecipitated with anti-p55/cdc20 and blotted with anti-BubR1 or anti-Mad2. (C) Increase in BubR1 activity induced by TAp73 overexpression. HeLa cells overexpressing TAp73 or ΔNp73 isoforms were treated and immunoprecipitated as in A and B. (D) Decreased TAp73 correlates with reduced SAC activity. Levels of phospho-BubR1 (P) and phospho-histone H3 (p-histh3) (representing SAC activity) were determined by Western blotting of HeLa cells treated with either control (siC) or TAp73 siRNA and subjected to nocodazole for the indicated number of hours. (E) TAp73 potentiates BubR1 kinase activity. HeLa cells overexpressing control, TAp73α, TAp73β, ΔNp73β, or p53 DNA were assayed for BubR1 kinase activity using histone-H1 as the substrate.