Fig. 2.

Confocal microscopy imaging of the yeast–phage interaction. Yeast cells displaying Z13e1 scFv were stained with anti-c-myc-Alexa Fluor 647 to visualize the presence of scFv on the cell surface (scFv-APC pseudocolored red). Binding of TJ1D phage to yeast cells was visualized by using an anti-phage antibody and Zenon-PE (phage-PE, pseudocolored green). For yeast cells binding to monomeric soluble protein, biotinylated HIV gp41 was visualized with streptavidin-PE (gp41-PE, pseudocolored green).