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Interaction of yeast and phage is specific to the displayed proteins. Yeast cells displaying Z13e1 scFv were labeled with anti-c-myc-Alexa Fluor 647 to visualize the presence of scFv on the cell surface (x axis). Phage binding to yeast cells was visualized by using an anti-phage antibody and Zenon-PE (y axis). (A) Phage TJ1N. (B) Phage TJ1D.
