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. 2009 Jan 12;106(3):888–893. doi: 10.1073/pnas.0807030106

Fig. 4.

Fig. 4.

Aag overexpression reverts the alkylation resistance of Aag mutant retinas. (A) Representative photomicrographs of retinas from Aag−/− mice or Aag−/− animals overexpressing the mouse Aag cDNA 7 days after treatment with 75 mg/kg of MMS. (Bars: 100 μm.) (B) (Left) Aag glycosylase activity on Hx–containing DNA on wild-type retinas, Aag−/− retinas, and retinas from Aag−/− animals overexpressing the mouse Aag cDNA (Aag−/− Tg+). − represents no protein control, and + indicates reaction in the presence of purified human AAG protein. (Right) Histogram plots quantifying the amount of glycosylase activity for the gels shown in Left. Activity is expressed as fmol Hx per unit released per μg of protein. The Aag DNA glycosylase assays were performed as previously described (21). Error bars equal SD.