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. 2009 Jan 13;6(1):e1000010. doi: 10.1371/journal.pmed.1000010

Figure 8. Western Blot Analysis Reveals HMGB1 Is Released from GL26, LLc1, GL261, and B16-F10 Cell Lines Following Treatment with Ad-TK (+GCV), Irradiation, or Temozolomide (TMZ).

Figure 8

(A) LLc1, GL261, or B16-F10 cells were infected with Ad-TK or Ad-0 at an MOI = 500. Noninfected cells were used as control. Ad-TK treated cells were also incubated with 25 μM GCV. 48 h later, supernatant was collected and HMGB1 release was assessed by western blotting.

(B) GL26, LLc1, GL261, or B16-F10 cells were irradiated with 20 Gy for 30 min. Nonirradiated cells were used as control. 72 h later, supernatant was collected and HMGB1 release was assessed by western blotting.

(C) GL26, LLc1, GL261, or B16-F10 cells were incubated with temozolomide (TMZ) (200 μM). Control cells were incubated without TMZ (mock). 48 h later, supernatant was collected and HMGB1 release was assessed by western blotting.