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Generation of B-cell precursors in mixed colonies formed on C166 cells. Nonadherent cells (103–104) isolated from secondary cocultures of YS-HSCs with irradiated C166 cells were cultured on S17 bone marrow stromal cells with interleukin 7 for 14 days. Expression of B220 and surface-associated IgM on cultured cells was determined by flow cytometric analysis.
