Table 4.
In vivo reconstitution of BALB/C scid mice by in vitro-passaged YS-HSCs
| Cells | CD4− CD8+ | CD4+ CD8+ | CD4− CD8− | CD4+ CD8− | TCRαβ+ | B220+ sIgM+ | B220+ sIgM− | Mac-1+ H-2Kb+ | H-2Kb+ H-2Kd− |
|---|---|---|---|---|---|---|---|---|---|
| Thymus | |||||||||
| scid | 3.3 | 68.9 | 26.4 | 1.0 | 0.5 | ||||
| + YS | 25.6 | 30.8 | 17.3 | 26.8 | 69.7 | ||||
| Spleen | |||||||||
| scid | 0.7 | 0.0 | 98.3* | 0.8 | 0.9 | 0.03 | 0.6 | 0.5 | 0.1 |
| + YS | 4.4 | 0.2 | 69.2* | 27.2 | 33.8 | 16.2 | 1.6 | 15.6 | 74.7 |
| PBL | |||||||||
| scid | 0.6 | 0.3 | 97.2 | 0.7 | 7.3 | 0.4 | 1.1 | 0.8 | 2.3 |
| + YS | 13.7 | 0.8 | 53.2 | 32.7 | 55.6 | 35.2 | 6.3 | 20.1 | 80.3 |
Nonadherent cells (2 × 104) from two-passage expansion of AA4.1+, WGA+, nonadherent, density <1.077 C57BL/6 yolk sacs were injected into irradiated BALB/c scid mice. Irradiated, unreconstituted scid mice served as controls. Cells from thymus, spleen, and peripheral blood were analyzed 12 weeks after transfer. Data are presented as % of total cells recovered. PBL, peripheral blood lymphocyte; TCR, T-cell receptor; sIgM, surface-associated IgM.
*
Number includes all lymphocytes, identified and gated on the basis of forward and side scatter signals.