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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: J Immunol. 2009 Jan 15;182(2):969–979. doi: 10.4049/jimmunol.182.2.969

Figure 2.

Figure 2

Analysis of Ag recognition in vivo by adoptively transferred, CFSE-labeled naïve βgalTCR T cells. (A) Representative FACS analysis of Vβ10+ lymphocytes recovered from recipient mice 10 days post-transfer for CFSE dilution and the indicated cell surface molecules. The percentage of lymphocytes having a resting/naïve (R) or an activated (A) phenotype is indicated. B10.A + Ag = 100 μg βgal peptide given ip to B10.A recipient mice three days post-T cell transfer. (B) Analysis of Ag specificity. FACS analysis of Vβ10+ lymphocytes from spleens of B10.A mice that received 100 rads irradiation prior to transfer of naive, CFSE-labeled βgalTCR T cells. Cells were harvested 14 days post-transfer. Recipients were given ip injections of saline (No Ag); 1 mg OVA; or 300 μg native βgal three days post-T cell transfer.