Skip to main content
. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Mol Cell. 2008 Sep 26;31(6):918–924. doi: 10.1016/j.molcel.2008.09.002

Figure 4. TRAF6 is required for the TGF-β-induced apoptosis and EMT.

Figure 4

(A–B) Effect of transfected TRAF6 and Smad3 siRNA on the apoptosis of AML12 cells. (A) Cell death was measured with the Cell Death Elisa kit after treating cells with or without TGF-β for 24 hours. (B) Caspase-3 activation was analyzed by Western blot. (C–D) Effect of transfected TRAF6 and Smad3 siRNA on EMT in NMuMG cells after treated with or without TGF-β for 48 hours. The cells were fixed and stained for F-actin (C) or subjected to Western analysis of E-cadherin and N-cadherin expression (D). Quantitations of E-cadherin and N-cadherin levels relative to γ-tubulin are shown at bottom, open bar: −TGF-β; solid bar: +TGF-β. (E) TGF-β-induced Smad2 and Smad3 activation in AML12 cells in the presence or absence of transfected TRAF6 siRNA. (F, G) (CAGA)12-Luc reporter assay of TGF-β responses in Hep3B cells that were transfected with siRNAs or TRAF6 expression plasmid. The ligand treatment was for 24 hours. All bar graphs are displayed as mean ± standard deviation.