Figure 5. Effect of diphenyleneiodinium chloride (DPI) on H₂O₂ production by BAE mitochondria as affected by mitoquinone (mQN) at different doses of rotenone (Rot).

Mitochondria were fueled by 5 mM glutamate plus 1 mM malate and incubated as in figure 4. Panel A) Mitochondria were incubated in respiratory buffer with no additions. Panel B) Addition of 5 µM DPI. Panel C) Mitochondria were incubated in chloride free respiratory buffer (gluconate substituted for chloride) with no additions. Panel D) Addition of 5 µM DPI in chloride free buffer. Data represent mean±SE, n = 10 in panels A and B, n = 6 in panels C and D. * p<0.05, † p<0.01, ≠ p<0.001 for mitoquinone compared to absence of the compound. Data were analyzed by 2-way ANOVA (factors mQN or Rot). Interaction was significant in panels A, B, and D.