Table 3.
FTIRI Parameters Measured in Images and Co-added Spectra of Dspp-/- and Dspp+/+ Mice Femora
| A. Cortical Bone | |||||||
|---|---|---|---|---|---|---|---|
| Parameter | 5 wk Dspp+/+ n=6 | 5 wk Dspp-/- n=5 | 7 mo Dspp+/+ n=7 | 7 mo Dspp-/- n=7 | 9 mo Dspp+/+ n=9 | 9 mo Dspp-/- n=6 | |
| Min/Mat | (imaging) | 7.0
(1.4) |
7.4
(0.4) |
7.5
(0.7) |
6.7
(0.4) |
6.8
(0.7) |
7.1
(0.6) |
| (co-added) | 7.3
(0.1) |
7.6
(0.7)* |
7.2
(0.8) |
7.5
(1.2) |
7.0
(0.2) |
6.9
(0.3) |
|
| CO3/PO4 | (imaging) | 0.0087
(0.0006) |
0.0068*
(0.0016) |
0.0066
(0.0004) |
0.0064
(0.001) |
0.0080
(0.0006) |
0.0077
(0.0008) |
| (co-added) | 0.0060
(0.0008) |
0.0048
(0.0002) * |
0.0069
(0.0009) |
0.0072
(0.0009) |
0.0067
(0.0005) |
0.009
(0.001) |
|
| Crystallinity | (imaging) | 1.15
(0.02) |
1.18
(0.03) |
1.12
(0.07) |
1.15
(0.06) |
1.13
(0.04) |
1.09
(0.03) |
| (co-added) | 1.090
(0.004) |
1.21
(0.03)* |
1.17
(0.03) |
1.18
(0.05) |
1.21
(0.03) |
1.16
(0.02)* |
|
| (curvefit) | 1.04
(0.14) |
1.19
(0.06)* |
1.07
(0.08) |
1.22
(0.08)* |
1.18
(0.02) |
1.16
(0.04) |
|
| XLR | (imaging) | 2.85
(0.10) |
4.0
(0.3)** |
3.4
(0.1) |
3.1
(0.1) |
3.2
(0.2) |
3.0**
(0.1) |
| (co-added) | 2.40
(0.10) |
4.13
(0.3)* |
3.9
(0.3) |
4.1
(0.4) |
3.91
(0.06) |
4.0
(0.3) |
|
| Acid P | (curvefit) | 1.96
(0.09) |
1.15
(0.40)* |
1.4
(0.5) |
2.0
(0.7) |
3.0
(1.3) |
0.7
(0.2)* |
| B. Trabecular Bone | |||||||
| Parameter | 5 wk Dspp+/+ n=6 | 5 wk Dspp-/- n=5 | 7 mo Dspp+/+ n=7 | 7 mo Dspp-/- n=7 | 9 mo Dspp+/+ n=9 | 9 mo Dspp-/- n=6 | |
| Min/Mat | (imaging) | 5.52
(1.11) |
5.16
(0.75) |
4.84
(1.01) |
5.70
(0.83) |
4.50
(0.87) |
4.86
(0.73) |
| (co-added) | 7.05
(0.75) |
5.73
(0.25)* |
5.22
(0.78) |
6.13
(0.59) |
5.23
(0.44) |
5.01
(0.50) |
|
| CO3/PO4 | (imaging) | 0.0079
(0.0009) |
0.0069
(0.0022) |
0.0067
(0.0013) |
0.0052**
(0.0007) |
0.0088
(0.0040) |
0.0066
(0.0009) |
| (co-added) | 0.0045
(0.0010) |
0.0070
(0.0011)* |
0.0063
(0.0009) |
0.0056
(0.0003) |
0.0074
(0.0011) |
0.0089
(0.0014) |
|
| Crystallinity | (imaging) | 1.080
(0.001) |
1.08
(0.04) |
1.07
(0.01) |
1.15**
(0.02) |
1.09
(0.05) |
1.05
(0.05) |
| (co-added) | 1.058
(0.006) |
1.081
(0.048) |
1.132
(0.078) |
1.172
(0.029) |
1.134
(0.045) |
1.095
(0.040) |
|
| Curvefit | 0.99
(0.11) |
1.35
(0.24)* |
1.50
(0.30) |
1.44
(0.20) |
1.11
(0.06) |
1.34
(0.11)* |
|
| XLR | (imaging) | 2.87
(0.29) |
3.80**
(0.20) |
2.72
(1.05) |
2.46
(0.56) |
3.59
(0.34) |
3.44
(0.47) |
| XLR | (co-added) | 2.42
(0.16) |
4.17
(0.48)* |
3.28
(0.22) |
3.63
(0.25)t |
3.64
(0.41) |
3.64
(.40) |
| Acid P | Curvefit | 2.30
(0.70) |
2.30
(.50) |
2.41
(0.60) |
3.06
(0.70) |
1.73
(0.37) |
2.31
(0.40)* |
Mean (standard deviation) –*p<0.05 (n=5) Comparison to Dspp+/+ of same age,
*
p<0.05,
**
p<0.01,
Min/Mat = peak area ratio of integrated areas of phosphate/amide I bands
CO3/PO4 = peak area ratio of integrated areas of carbonate (850-890 cm-1)/phosphate bands
Crystallinity = peak height intensity of subbands at 1030 cm-1(stoichiometric apatite) / 1020 cm-1 (non-stoichiometric apatite)
XLR = collagen cross link ratio; peak height intensity ratio of subbands at 1660 cm-1 /1683 cm-1