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. 1996 Dec 10;93(25):14788–14794. doi: 10.1073/pnas.93.25.14788

Figure 2.

Figure 2

PCR identification of apo A-II-deficient mice, Northern blot analysis of mouse liver RNA, and SDS/PAGE analysis of mouse HDL. (A) The wild-type allele was identified by the PCR primer pair MAII6u and MAII2l, which gave a 0.35-kb band. The mutant allele was identified by the PCR primer pair MAII4l and Neo901, which gave a 0.7-kb band. (B) RNA was isolated from mouse liver. The Northern blot on the left was hybridized with mouse apo A-I cDNA probe; the Northern blot on he right was hybridized with mouse apo A-II cDNA probe. Apo A-II is completely absent from the apo A-II knockout lane. The mRNA levels of heterozygous are only half of that of controls. Apo A-I mRNA levels were unchanged among the three genotypes. +/+, +/−, and −/− represent control littermates, heterozygous-deficient mice, and homozygous apo A-II-deficient mice, respectively. (C) HDL was isolated by sequential ultracentrifugation and HDL from equal amounts of plasma was subjected onto SDS/PAGE (10–25% gradient). The gel was stained with Coomassie blue R-250. There was no detectable apo A-II in the homozygous-deficient mouse HDL.