Figure 3.

FPLC fractionation of plasma from apo A-II-deficient and control mice on chow diet. Two hundred microliters of fasted, pooled, and filtered mouse plasma was applied to two Superose 6 column (Pharmacia) connected in series. The lipoproteins were eluted at a constant flow rate of 0.3 ml/min with 1 mM sodium EDTA and 0.15 M NaCl. Fractions of 0.5 ml were shown as mg/dl in each fraction.