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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Bone. 2008 Jun 6;43(4):689–699. doi: 10.1016/j.bone.2008.05.022

Figure 1. HPSE is present in the Pc and COJ of developing mouse limb.

Figure 1

Panel A shows hematoxylin and eosin counterstaining of the cryosection shown in (B). (B) Hpse mRNA (orange and yellow) was detected by in situ hybridization, in the periosteum (Po) and perichondrium (Pc), hypertrophic zone (Hz), and chondro-osseous junction (COJ) and mineralized tissue (MT) of day 18.5 embryos. (C) A cryosection of mouse hindlimb was analyzed by indirect immunofluorescence using a mouse monoclonal anti-human HPSE antibody as described in “Materials and Methods.” Positive HPSE immumostaining is indicated by green fluorescence in the Pc, Po, COJ, and MT. The section was double labeled with anti-perlecan/HSPG2, the major HSPG in the matrix of developing endochondral bone (red). (D) Close up view of HPSE immunostaining (green) in the Pc at the PHz/Hz boundary shown with perlecan/HSPG2 double stain (red). (E) HPSE staining of the COJ. No specific signal was observed using isoform matched normal mouse IgG under similar conditions used as a negative control (not shown). Arrows highlight areas of high HPSE/Hpse expression.