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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Bone. 2008 Jun 6;43(4):689–699. doi: 10.1016/j.bone.2008.05.022

Figure 2. PI-88 reduces growth of cultured metatarsals.

Figure 2

Freshly dissected mouse metatarsals were cultured for 5 days as described in “Materials and Methods.” Cultures received either 100 or 500 µg/ml PI-88, or no treatment. Metatarsals were, on average, 3.53 mm in length at the initiation of the culture period. (Panels A, C) In the absence of PI-88, metatarsal bones increased in total length by 21.5% over the culture period. No change was observed at the lower dose (100 µg/ml) of PI-88. At the higher dose, a significant (p < 0.05) reduction in bone lengthening, expressed as a percent, was seen both by calculation of the mean and the median. (Panel B) Immunohistochemical analysis of sections of metatarsal bone treated with PI-88 (500 µsg/ml) showed a loss of cells in the proliferating zone (Pz) and a concomitant increase in cells in the hypertrophic zone (Hz). These features are consistent with limb shortening. (Panel C) Mean and median growth was assessed at two doses of PI-88 (n ≥ 8). The data shown is compiled from two independently performed experiments in which five bones were used in each treatment group (30 bones total).