Table I.

PCR Primers Used In These Studies

Gene	Forward Primer	Reverse Primer	Product Size (bp)	Annealing Temperature (°C)	Reference
Aggrecan/Agg	5’-CCAAGTTCCAGGGTCACTGT-3’	5’-TCTAGCATGCTCCACCACTG-3’	398	60	[84]
Type II Collagen/ColII	5’-TCATCGAGTACCGATCACAGA-3’	5’-GTTCGGGGGTTTTACAAGAAG-3’	130	55–60	[56]
Type X Collagen/ColX	5’-TAGGAGCTAAAGGAGTGCCT-3’	5’-TCACCCTTAGATCCAGGGAAT-3’	124	55–60	[56]
Hpse	5’-TTTGCAGCTGGCTTTATGTG-3’	5’-ACTGGTTTCCTGAACAACGG-3’	349	57.8	new
Osteocalcin/Ocn	5’-AAGTCCCACACAGCAGCTTG-3’	5’-AGCCGAGCTGCCAGAGTTTG-3’	400	65	[85]
ActB	5’-GTGGGCCGCTCTAGGCACCAA-3’	5’-CTCTTTGATGTCACGCACGATTTC-3’	502	55–60	new
Hpse (for QPCR)	5’-TGTCCTGAACCTCCATAATGTC-3’	5’-TACGTATCCACTGGTTTCCTGA-3’	79	60	new
ActB (for QPPR)	5’-AAATCGTGCGTGACATCAAAGA-3’	5’-GCCATCTCCTGCTTCGAAGTC-3’	80	60	[56]