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. 1996 Dec 10;93(25):14827–14832. doi: 10.1073/pnas.93.25.14827

Figure 3.

Figure 3

Detection of colon carcinoma cells in blood of patients using RT-PCR and GCC-specific nested primers. Total RNA was extracted from a 1-ml aliquot of blood cells, mRNA-purified, and used for RT-PCR, using GCC-specific nested primers, as described. Border lanes (M) indicate size markers (200–1000 bp). Arrow indicates the predicted size of the amplification product for human GCC (hGCC; ≈250 bp). Lane 1, normal volunteers (n = 10); lane 2, patients with a colonic adenomatous polyp (n = 6); lane 3, patient with an acute exacerbation of inflammatory bowel disease (n = 1); lane 4, patient with adenocarcinoma of the lung (n = 1); lane 5, patient with adenocarcinoma of the breast (n = 1); lane 6, patient with adenocarcinoma of the ovary (n = 1); lane 7, patient with lymphoma (n = 1); lane 8, patients with pancreatic carcinoma (n = 6); lanes 9–11, patients with Dukes A colorectal cancer; lanes 12–16, patients with Dukes B colorectal cancer; lanes 17–20, patients with Dukes C colorectal cancer; and lanes 21–30, patients with Dukes D colorectal cancer.