Figure 6.

Inhibition by CD68 mAbs of specific binding and uptake of ¹²⁵I-labeled OxLDL (5 μg/ml) by THP-cells. THP-1 cells were treated with PMA for 4 days after which binding at 4°C (Left) and uptake at 37°C (Right) were measured in the absence or presence of a 40- to 50-fold excess unlabeled OxLDL, EBM-11 (n = 5), KP-1 (n = 4), or an isotype-matched control IgG. Cell-bound and cell-associated radioactivity was assessed after 4–5 hr and were ≈260 and ≈800 ng of OxLDL per mg of cell protein, respectively. Nonspecific OxLDL binding and uptake (≈131 and 360 ng/mg of cell protein, respectively) were subtracted from all values to yield specific binding values. Activities in the presence of control IgG are taken as 100% (see Results). Data represent mean ± SD.