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T/P associates with signaling molecules. Ba/F3–PDGFβR and Ba/F3 T/P cells were lysed and immunoprecipitated with anti-PDGFβR antisera. Immunoprecipitates were separated by SDS/PAGE, transferred to nitrocellulose, and blotted with the indicated antibodies. Ba/F3–PDGFβR were either deprived of IL-3 for 4 hr (−) or stimulated with 100 ng of recombinant human PDGF B for 10 min at 37° (+).
