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. 1996 Dec 10;93(25):14851–14856. doi: 10.1073/pnas.93.25.14851

Figure 2.

Figure 2

pGEM and pEXI reacted with surface biotinylated PMN and HL-60 cells. PMN or HL-60 cells were lysed with Triton X-100 and mixed with bacteria. After incubation the bacteria were recovered by centrifugation, lysed, the lysates subjected to SDS/PAGE and transferred to Immobilon-P membrane. Biotinylated proteins were detected by staining with extravidin-conjugated peroxidase and chemiluminescence. PMN total lysate (lane 1) and HL-60 cell total lysate (lane 2). Proteins recovered from PMN lysate with pEXI (lane 3) and pGEM (lane 4). Proteins recovered from HL-60 cells lysate with pEXI (lane 5) and pGEM (lane 6). pEXI bind a 30-kDa band specifically from PMN lysates (lane 3). The 23-kDa protein recognized by extravidin is probably the biotin carboxy carrier protein found in E. coli (26).