TABLE 3.
Phylogenetic affiliations and frequency of isolates and clone libraries
| Phylogenetic grouping | Arctic
|
Antarctic
|
Nearest phylogenetic relativea | % Identical 16S rRNAb | ||||
|---|---|---|---|---|---|---|---|---|
| No. of isolates | No. of
clones
|
No. of
isolates
|
No. of clones
|
|||||
| ARKXIII/2 | ARKXV/1 | ARKXVI/2 | ANTV/2 | ANTXI/4_13 | ANTXI/4_14 | |||
| Bacteria | 115 | 86 | 106 | 87 | 103 | 95 | ||
| α-Proteobacteria | ||||||||
| Roseobacter | 1 | 3 | 4 | Antarctic quartz stone sublithic isolate QSSC9-5 (AF170750) | 93.0-97.8 | |||
| 2 | 1 | Adriatic Sea isolate AS-26 (AJ391187) | 97.2-99.7 | |||||
| 5 | 6 | 4 | Roseobacter sp. strain Shippagan (AF100168) | 98.1-98.9 | ||||
| 2 | Roseobacter litoralis (X78312) | 98.0 | ||||||
| 3 | 1 | 4 | North Atlantic continental slope isolate EI-2 (AF254103) | 96.9-98.0 | ||||
| 29 | 7 | 5 | 8 | 1 | Octadecabacter arcticus (U73725) | 97.0-99.0 | ||
| 5 | Ligurian Sea isolate SRF3 (AJ002565) | 98.1 | ||||||
| 7 | Mediterranean Sea clone MED-6 (AF025549) | 97.0-98.3 | ||||||
| 1 | Nankai Trough clone NKB7 (AB013259) | 95.1 | ||||||
| Sphingomonas | 2 | Antarctic seawater isolate SW54 (U85838) | 96.5 | |||||
| 1 | Erythrobacter longus (M59062) | 97.8 | ||||||
| β-Proteobacteria | ||||||||
| 1 | Soil ultramicrobacterium strain ND5 (AB008506) | 97.8 | ||||||
| γ-Proteobacteria | ||||||||
| Shewanella | 1 | 8 | Shewanella frigidimarina ACAM584 (U85902) | 99.3-99.7 | ||||
| Psychromonas | 3 | 3 | Psychromonas antarctica (U14697) | 97.7-99.5 | ||||
| Colwellia | 5 | 9 | 1 | Colwellia psychroerythraea IC064 (U85842) | 98.2-98.9 | |||
| 15 | 21 | 6 | 37 | 40 | Colwellia sp. strain ANT5-9 (AF19350) | 97.4-99.6 | ||
| 9 | Colwellia rossensis ACAM608 (U14589) | 99.3-99.7 | ||||||
| 4 | 4 | 5 | 7 | 8 | Colwellia-like sp. strain IC169 (AF001376) | 98.0-98.2 | ||
| Glaciecola | 5 | 19 | 23 | 25 | 15 | Gas vacuolate sea ice bacterium strain 214.6 (GVstr214.6 [U73724]) | 97.6-98.7 | |
| 10 | 2 | 2 | 4 | Glaciecola punicea ACAM 611T (U85853) | 97.0-98.3 | |||
| 4 | 5 | 4 | Glaciecola pallidula ACAM 615T (U85854) | 98.6-98.7 | ||||
| Pseudoalteromonas | 2 | Pseudoalteromonas antarctica (X98336) | 99.7-100 | |||||
| Methylophaga | 1 | Methylophaga marina (X95459) | 94.5 | |||||
| Psychrobacter | 4 | 1 | 2 | 3 | 1 | 3 | Psychrobacter glacincola IC084 (U85876) | 98.1-99.7 |
| 1 | Psychrobacter marincola (PMA309941) | 99.6 | ||||||
| 3 | Psychrobacter sp. strain St1 (AF260715) | 98.0-99.5 | ||||||
| Acinetobacter | 1 | Acinetobacter johnsonii (X89775) | 99.5 | |||||
| 1 | Acinetobacter lwoffii (U10875) | 98.8 | ||||||
| Pseudomonas | 4 | Pseudomonas sp. strain ACAM213 (U85868) | 98.3-100 | |||||
| 2 | Arctic seawater bacterium R7366 (UBA293826) | 99.4 | ||||||
| Marinobacter | 8 | 13 | 1 | 1 | Marinobacter sp. strain IC022 (U85863) | 97.1-99.0 | ||
| 18 | Marinobacter sp. strain DS40M8 (AF199440) | 95.0-99.7 | ||||||
| 1 | Marinobacter hydrocarboniclasticus (AB021372) | 91.2 | ||||||
| Oceanospirillum | 2 | 11 | 2 | Oleispira antarctica (AJ426420) | 98.4-99.3 | |||
| 1 | 1 | Marinomonas protea (AJ238597) | 96.0-99.4 | |||||
| Halomonas | 2 | Halomonas variabilis strain SW04 (U85873) | 98.6-99.8 | |||||
| 1 | Gamma Proteobacterium MBIC3958 (AB020600) | 95.1 | ||||||
| 1 | Antarctic shelf clone MERTZ_2CM_20 (AF424059) | 97.0 | ||||||
| Terredinibacter | 2 | Arctic sea ice clone ARCTIC_ICE-12 (AF277547) | 98.1 | |||||
| NOR5c | 1 | North Sea clone KTc1119 (AF235120) | 95.1 | |||||
| CF | Polaribacter filamentus (U73726) | 98.9-99.5 | ||||||
| Polaribacter | 2 | 1 | Arctic Ocean clone Arctic 96B-11 (AF354621) | 93.7 | ||||
| 1 | 3 | 8 | Polaribacter franzmannii (U14586) | 97.9-98.1 | ||||
| 1 | 4 | 2 | 7 | Polaribacter irgensii (M61002) | 99.1-99.3 | |||
| Cellulophaga | 3 | 3 | Mesocosm diatom bloom isolate 5.3.10 (AF367849) | 98.0-98.3 | ||||
| Flavobacterium | 3 | Flavobacterium xanthum IC001 (U85889) | 98.7-99.1 | |||||
| Salegentibacter | 7 | Salegentibacter salegens (M92279) | 97.9-99.1 | |||||
| Psychroflexus | 2 | 2 | Psychroflexus torquis ACAM623 (U85881) | 98.1-98.7 | ||||
| 16 | North Atlantic abyssal isolate AIII4 (AF254114) | 92.9-96.2 | ||||||
| Psychroserpens | 6 | Psychroserpens burtonensis ACAM188 (U62913) | 94.0-95.8 | |||||
| Cytophaga | 2 | 5 | Arctic Ocean clone Arctic96AD-4 (AF354619) | 96.8-98.1 | ||||
| 1 | Japan Trench clone JTB132 (AB015260) | 98.8 | ||||||
| 1 | Antarctic sea ice clone SIC.42370 (AF277483) | 93.8 | ||||||
| Green nonsulfur bacteria | 1 | Arctic Ocean clone Arctic 95A (AF355054) | 87.3 | |||||
| Actinobacteria | 2 | Nesterenkonia halobia (X80847) | 97.9 | |||||
| 1 | 1 | Soil ultramicrobacterium str. 121 (AB008511) | 97.8-98.0 | |||||
| 1 | Brachybacterium faecium (X91032) | 91.8 | ||||||
| 1 | Microbacterium sp. VKM Ac-2047 (AB042082) | 99.4 | ||||||
| 3 | Freshwater clone HTG6 (AF418963) | 91.7-92.0 | ||||||
| Total 16S rRNA phylotypes | 33 | 15 | 19 | 20 | 13 | 11 | ||
| Shannon index ± variance | 1.291 ± 0.002 | 1.065 ± 0.002 | 1.091 ± 0.001 | 1.127 ± 0.002 | 0.844 ± 0.002 | 0.812 ± 0.001 | ||
| Coverage (%) | 53.3 | 73.6 | 84.6 | 81.8 | ||||
a
In ARB database.
b
Estimated in ARB.
c
Reference 24.