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. 2003 Nov;69(11):6597–6604. doi: 10.1128/AEM.69.11.6597-6604.2003

FIG. 2.

FIG. 2.

Quantification of total bacterial 16S rRNA genes (A and B) and Nitrospira 16S rRNA genes (C and D) using real-time PCR in reactors operating at 2-, 5-, 10-, and 20-day SRTs. Separate extractions are indicated with black, white, and diagonal bars (Average ± standard deviation of three determinations). Targets are expressed in copies/liter (L) of mixed-liquor (A and C) or copies/microgram of DNA (B and D). The detection limits for the bacterial 16S rRNA and Nitrospira 16S rRNA genes were 3.6 × 109 copies/liter and 1.0 × 107 copies/liter, respectively, and 1.5 × 107 copies/μg of DNA and 4.2 × 104 copies/μg of DNA, respectively.