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. 2003 Nov;69(11):6620–6627. doi: 10.1128/AEM.69.11.6620-6627.2003

FIG. 3.

FIG. 3.

Determination of Blg41-60::Nuc antibody reactivity. (A) Competitive EIA. Anti-41-60 Blg-21R MAb, synthetic Blg41-60 labeled with AChE, and increasing concentrations of synthetic Blg41-60 (▪) or recombinant Blg41-60::Nuc (▴) were added to a microtiter plate that was previously coated with anti-mouse antibody. (B) Competitive ELISA. Blg-21R MAb labeled with AChE and increasing concentrations of Blg41-60 or Blg41-60::Nuc were added to microtiter plates that were previously coated with Blg41-60::Nuc. For both experiments, concentrations of both competitors are expressed as picomoles of Blg41-60 per milliliter. Data are means of duplicate determinations. Absorbance was measured at 414 nm.