FIG. 4.
Kinetics of Blg41-60::Nuc expression. L. lactis strain NZ9000(pSEC:Blg41-60:Nuc) was grown to an OD600 of 0.5, and then Blg41-60::Nuc expression was induced with nisin. Blg41-60::Nuc was assayed by competitive EIA at different times postinduction (1, 2, 3, 4, and 18 h) in supernatant (▪), cytoplasmic soluble proteins (▴), and cytoplasmic insoluble proteins (▾). Results are expressed as concentrations of Blg41-60::Nuc. Values given represent the means ± standard deviations for three independent experiments.