Table 2. TaqMan probes.
| Entrez Gene ID | Gene | TaqMan probe ID | Amplicon length |
|---|---|---|---|
| Axon guidance genes | |||
| 22854 |
NTNG1 |
Hs01552822_m1 |
61 |
| 8829 |
NRP1 |
Hs00826129_m1 |
97 |
| 91584 |
PLXNA4 |
Hs00326001_m1 |
89 |
| 10154 |
PLXNC1 |
Hs00194968_m1 |
72 |
| 223117 |
SEMA3D |
Hs00380877_m1 |
97 |
| 56920 |
SEMA3G |
Hs00928864_m1 |
106 |
| 10505 |
SEMA4F |
Hs00188642_m1 |
64 |
| 137970 |
UNC5D |
Hs00369888_m1 |
83 |
| Regulators of Angiogenesis | |||
| 4879 |
NPPB |
Hs00173590_m1 |
82 |
| 5176 |
PEDF |
Hs01106937_m1 |
84 |
| 7422 |
VEGFA |
Hs00900057_m1 |
81 |
| Other | |||
| 2247 |
FGF2 |
Hs00266645_m1 |
82 |
| 2264 |
FGFR4 |
Hs00242558_m1 |
74 |
| Control | |||
| 2597 | GAPDH | Hs99999905_m1 | 122 |
QRT–PCR amplification of genes not listed in Table 1 was performed using TaqMan probes (Applied Biosystems) and normalized relative to a GAPDH reference gene. GAPDH gene expression was also measured using a TaqMan probe.