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. Author manuscript; available in PMC: 2009 Jan 13.
Published in final edited form as: Cytometry B Clin Cytom. 2008 Sep;74(5):319–329. doi: 10.1002/cyto.b.20421

Figure 2.

Figure 2

Identification of monocytes in whole blood samples requires CD14-PE thresholding and precludes single color compensation controls. Side scatter vs. forward scatter plots are show for whole blood and PBMC samples with thresholding on forward scatter (Panels A and B) or CD14-PE fluorescence (Panels C and D). Because monocyte data enrichment in whole blood samples cannot be done with SSC vs. FSC plots (Panel A), the Calibrite bead compensation method is used as a surrogate for YO-PRO-1 compensation controls in whole blood samples already containing CD14-PE. Using the Calibrite bead adjusted setup method, Panels E and F show that data restriction on viable CD14-PEpos/PIneg events is just as easy in whole blood as it is in PBMC samples. Finally, Panels G and H show the effects of bead-determined compensation on PE versus YO-PRO-1 analysis when restricted to live events in whole blood and PBMC samples respectively. The unstimulated YO-PRO-1 labeled monocytes (black) are overlayed with stimulated monocytes (gray).