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. Author manuscript; available in PMC: 2009 Jan 13.
Published in final edited form as: Cytometry B Clin Cytom. 2008 Sep;74(5):319–329. doi: 10.1002/cyto.b.20421

Figure 4.

Figure 4

Comparative histograms of the YO-PRO-1 fluorescence of monocytes stimulated with BzATP in two donors: one low responder and one high responder. Data was acquired on the same sample on three different flow cytometers: FACScan, FACSCalibur, and the LSRII. The instruments were set using two sets of target values as described. The FACScan and FACSCalibur used the same values while the LSRII used a different set of values. Median intensitites of live monocytes are given for each instrument, with the values for the BzATP stimulated and the unstimulated samples as well as the MFI ratio presented in the insets.