TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| Cycloclasticus sp. strain A5 | Degrades PAHs. such as naphthalene. phenanthrene. biphenyl. etc. | 24 |
| E. coli JM109 | recA1 relA1 thi-1 Δ(lac-proAB) gyrA96 hsdR17 endA1 supE44 (rk− mk−) [F7 traD36 proAB lacIrZΔM15] | 60 |
| E. coli XL1-Blue | hsdR17 supE44 recA1 endA1 gyrA46 thi-1 relA1 lac/F′ [proAB+ lacIqlacZΔM15::Tn10 (Tetr)] | Stratagene |
| E. coli XL1-Blue MRA | Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA96 gyrA1 relA1 lac | Stratagene |
| Plasmids | ||
| Lorist6 | Kmr: 5.28-kb cosmid vector | 15 |
| pBluescript II SK+ | lacZ Apr: 2.96-kb cloning vector | Stratagene |
| pH1a | Kmr Lorist6::11-kb Sau3A1 fragment from A5 containing phnA1A2A3A4 | This study |
| pH1b | Kmr Lorist6::11-kb Sau3A1 fragment from A5 containing phnA1A2A3A4 | This study |
| pPhnA | Apr: pBluescript:: ring-hydroxylating dioxygenase gene phnA1A2A3A4 from A5 | This study |
| pPhnC | Apr: pBluescript:: extradiol dioxygenase gene phnC from A5 | This study |