TABLE 3.
Properties of the phn genes identified on the 10.5-kb fragment of A5
| Gene | Function | Deduced molecular mass (kDa) | Protein with similar sequence | % Identity (no. of residues) | Organism | Accession no. |
|---|---|---|---|---|---|---|
| orf1 | PdxA homolog | 34 | Orf1158 | 56 (319) | Sphingomonas aromaticivorans F199 | NP_049207 |
| PdxA | 35 (328) | Bacillus halodurans C-125 | NP_241670 | |||
| PdxA | 34 (322) | Fusobacterium nucleatum ATCC 25586 | NP_603133 | |||
| phnA1b | ISP α subunit | 48 | AhdA1d | 60 (424) | Sphingomonas sp. strain P2 | BAC65433 |
| BphA1d | 60 (426) | Sphingomonas aromaticivorans F199 | NP_049206 | |||
| AhdA1c | 48 (420) | Sphingomonas sp. strain P2 | BAC65426 | |||
| PhnA1 | 22 (460) | Cycloclasticus sp. strain A5 | AB102786 | |||
| phnA1 | ISP α subunit | 52 | BphA1f | 64 (452) | Sphingomonas aromaticivorans F199 | NP_049062 |
| DbtAc | 51 (443) | Burkholderia sp. strain DBT1 | AAK62353 | |||
| PhnAc | 52 (416) | Alcaligenes faecalis AFK2 | BAA76323 | |||
| phnA2 | ISP β subunit | 21 | BphA2f | 52 (173) | Sphingomonas aromaticivorans F199 | NP_049061 |
| ThnA2 | 39 (177) | Sphingopyxis macrogoltabida TFA | AAN26444 | |||
| BphA2 | 33 (171) | Comamononas testosteroni TK 102 | BAC01053 | |||
| phnC | Extradiol dioxygenase | 34 | BphC | 66 (289) | Sphingomonas paucimobilis Q1 | P11122 |
| DmdC | 64 (289) | Sphingomonas paucimobilis TZS-7 | BAB07894 | |||
| BphC | 64 (289) | Sphingomonas aromaticivorans F199 | NP_049210 | |||
| trpB | Tryptophan synthase β subunit | 42 | TrpB | 57 (388) | Thermoanaerobacter tengcongensis MB4T | NP_623178 |
| TrpB-2 | 56 (380) | Pyrococcus furtosus DSM 3638 | NP_579435 | |||
| TrpB | 56 (391) | Brucella suis 1330 | NP_699085 | |||
| orf7 | Unknown | 26 | No obvious homolog | |||
| phnA4 | Reductase | 38 | TouF | 50 (313) | Pseudomonas stutzeri OX1 | CAA06659 |
| PhnAa | 47 (338) | Alcaligenes faecalis AFK2 | BAA76321 | |||
| ThnA4 | 47 (342) | Sphingopyxis macrogoliabida TFA | AAN26446 | |||
| phnA3 | Ferredoxin | 11 | BphA3 | 63 (104) | Sphingomonas aromaticivorans F199 | NP_049211 |
| PhnR | 63 (104) | Sphingomonas chungbukensis | AAC95320 | |||
| NsaA3 | 59 (103) | Sphingomonas sp. strain BN6 | AAB06726 | |||
| phnD | Isomerase | Orf8 | 64 (115) | Burkholderia sp. strain DBT1 | AF96187 | |
| NsaD | 61 (115) | Sphingomonas sp. strain BN6 | AAD45416 | |||
| NahD | 58 (115) | Sphingomonas aromaticivorans F199 | NP_049214 |