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. 1996 Dec 10;93(25):14868–14872. doi: 10.1073/pnas.93.25.14868

Figure 3.

Figure 3

In vitro binding of proteins to fragments of the ech-42 promoter. (A) Schematic representation of the method used to prepare the DNA fragments for EMSA. PCR was performed with combinations of primers 221, E1, E2, and E3 as described. The final 221 plus E3 amplification product was sequenced and digested with SalI to obtain the two fragments used for EMSA (S1 and S2). (B) EMSA, using protein extracts of T. harzianum and two fragments of the ech-42 promoter region. Lanes: 1, free DNA; 2, DNA plus extract from a3; 3, DNA plus extract from a1; 4, DNA plus protein extracts from the mycelium of a T. harzianum–T. harzianum interaction; and 5, DNA plus protein extracts from mycelium of B. cinerea during a2.