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. 1996 Dec 10;93(25):14868–14872. doi: 10.1073/pnas.93.25.14868

Figure 4.

Figure 4

Demonstration of the involvement of Cre1 in protein–DNA binding during EMSA with protein extracts of T. harzianum and the ech-42 fragments S1 and S2. Two oligonucleotides, one containing a double Cre1-binding site (Cre1wt) and one containing the same site mutated and unable to bind Cre1 (Cre1mu), were used. (A) Lanes: 1, free DNA; 2, DNA plus protein extracts from a1 (see Fig. 1); 3–5, DNA plus protein extracts from a1 in the presence of a 5-fold (lane 3), 10-fold (lane 4), and 50-fold (lane 5) molar excess of Cre1wt; and 6 and 7, DNA plus protein extracts from a1 in the presence of a 50-fold (lane 6) and 100-fold (lane 7) molar excess of Cre1mu. (B) Lanes: 1, free DNA; 2, DNA plus protein extracts from a3 (see Fig. 1); 3 and 4, DNA plus protein extracts from a3 in the presence of a 50-fold (lane 3) and 100-fold (lane 4) molar excess of Cre1wt; and 5, DNA plus protein extracts from a3 plus a 100-fold molar excess of Cre1mu.