TABLE 2.

Primers used in this study

Primera	Sequence (5′ to 3′)	Reference
16S rRNA
(GC-)CYA371F	CCT ACG GGA GGC AGC AGT GGG GAA TTT TCC	27
CYA783R	GAC TAC (A/T)GG GGT ATC TAA TCC C(A/T)	27
(GC-)CSIF	G(T/C)C ACG CCC GAA GTC (G/A)TT AC	This study
rRNA-ITS
373R	CTA ACC ACC TGA GCT AAT	40
ITS3R	TAT GCA GTT TTC AAG GTT C	39
23S rRNA
ULR	CCT CTG TGT GCC TAG GTA TC	13, 26

^aF (forward) and R (reverse) refer to the primer orientation in relation to rRNA genes. A 40-nucleotide G-C clamp 5′-CGC CCG CCG CGC CCC GCG CCC GGC CCG CCG CCC CCG CCC C-3′ was added to the 5′ end of the forward primers indicated with (GC-).