TABLE 3.
Number and approximate sizes of the PCR products of strains from various cyanobacterial genera amplified with the primers described in this studya
| Strain | ITSa | ITSb | ITSc |
|---|---|---|---|
| Microcystis aeruginosa | 300 | 500 | 550 |
| Synechocystis sp. | 325 | 675 | |
| Synechococcus | 325 | 650 | 750 |
| Leptolyngbya | 275 | 500 | |
| Lyngbya sp. | 300 | 675 | |
| Microcoleus sp. | 300 | ND | 675 |
| Pseudanabaena catenata | 300 | 675 | 750 |
| Planktothrix prolifica | 300 | 550 | 525, 700 |
| Planktothrix aghardi | 300 | 550 | 525, 700 |
| Anabaena variabilis | 300 | 375, 600 | 500, 700 |
| Anabaenopsis arnoldii | 300 | 350, 650 | 450, 725 |
| Aphanizomenon flos-aquae | 300 | 400, 625 | 475, 700 |
| Aphanizomenon flos-aquae f. gracile | 300 | 400, 625 | 475, 700 |
| Cylindrospermopsis raciborskii | 300 | 350, 525 | 425, 600 |
| Nostoc sp. | 300 | 375, 650 | 500, 775 |
| Nodularia spumigena | 300 | 400, 700 | 575, 875 |
| Gloeotrichia sp. | 275 | 375, 675 | 475, 775 |
| Trichodesmium erythraeum | 300 | 550 | 675 |
| Prochlorothrix hollandica | 350 | 800 | 1050 |
a
Primer combinations are described in the text. The forward primers include a 40-nucleotide GC-clamp. PCR product sizes (in base pairs) were estimated from agarose gels. ND, not determined.