TABLE 1.
Abilities of PT7-traR and PtetR-traR strains to respond to different AHLsa
| Compound | β-Galactosidase activity
|
|||
|---|---|---|---|---|
| PT7-traR
|
Ptet-traR
|
|||
| 5 μM | 5 nM | 5 μM | 5 nM | |
| 3-Oxobutanoyl-HSL | 712.5 | 9.9 | 30.9 | 2.3 |
| 3-Oxopentanoyl-HSL | 1,418.2 | 402.9 | 932.6 | 2.5 |
| 3-Oxohexanoyl-HSL | 1,687.9 | 1,326.9 | 1,607.8 | 407.8 |
| 3-Oxoheptanoyl-HSL | 1,185.2 | 15.5 | 248.9 | 1.6 |
| 3-Oxooctanoyl-HSL | 1,907.9 | 1,767.2 | 1,702.5 | 920 |
| 3-Oxoundecanoyl-HSL | 1,509.2 | 1,143.7 | 1,466.1 | 423.8 |
| 3-Oxodocecanoyl-HSL | 1,476.3 | 1,294.5 | 1,255.7 | 91.5 |
| 4-Oxa-3-oxohexanoyl-HSL | 1,571.1 | 195.8 | 626.3 | 3.8 |
| Butanoyl-HSL | 279 | 23.1 | 19.8 | 2.2 |
| Pentanoyl-HSL | 1,170.8 | 169.8 | 362.7 | 2.9 |
| Hexanoyl-HSL | 1,316.9 | 37 | 672.8 | 2.4 |
| Heptanoyl-HSL | 1,678.7 | 1,481.8 | 957.4 | 279.8 |
| Octanoyl-HSL | 1,905.9 | 1,772.9 | 760.7 | 275.1 |
| Decanoyl-HSL | 1,781.6 | 37.1 | 467.3 | 2.2 |
| Dodecanoyl-HSL | 501.2 | 8 | 43.6 | 3 |
| 3-Hydroxynonanoyl-HSL | 1,796.3 | 1,683 | 1,487.5 | 437.6 |
| 3-Hydroxydodecanoyl-HSL | 1,602.6 | 197.9 | 216.4 | 6.9 |
| 2-Butenoyl-HSL | 73.8 | 8 | 10.4 | 2.3 |
| 2-Pentenoyl-HSL | 42.6 | 7.5 | 12.1 | 2.2 |
| 2-Hexenoyl-HSL | 1,672.1 | 346.3 | 766.6 | 5.2 |
| 2-Octenoyl-HSL | 1,653.1 | 1,303.8 | 1,114.7 | 58.9 |
| 2-Nonenoyl-HSL | 1,733.1 | 1,304.2 | 1,261.3 | 61.1 |
| 2-Decenoyl-HSL | 1,536.9 | 924.1 | 1,319.4 | 9.5 |
| 2-Butynoyl-HSL | 1,744.1 | 54.2 | 669.3 | 3 |
| 2-Hexynoyl-HSL | 1,576.1 | 18.4 | 285.6 | 1.7 |
| 5-Hexynoyl-HSL | 657.8 | 9.4 | 56.3 | 1.6 |
| 2-Octynoyl-HSL | 1,448.8 | 36.3 | 813.5 | 1.9 |
| 3-Oxo-7-octynoyl-HSL | 2,115.1 | 1,854.9 | 1,862.9 | 646.9 |
| 3-Oxo-11-octadecenoyl-HSL | 813.4 | 25 | 67.3 | 3.3 |
| Di-HSL-decandioate | 967 | 16.5 | 49.2 | 1.8 |
| Di-HSL-3,12-dioxotetradecandioate | 1,208.8 | 210.3 | 529.6 | 8.3 |
| p-Propylbenzoyl-HSL | 136 | 11.5 | 14.2 | 1.7 |
| O-Hexyl-N-HSL | 1,963.4 | 717.5 | 1,974.4 | 8.3 |
| HSL | 19.7 | 4.1 | 11.1 | 2.9 |
a
AHL analogs were provided at the indicated concentrations to cultures of KYC55(pJZ372)(pJZ384)(pJZ410) (left two columns of values) and WCF47(pCF218)(pCF372) (right two columns of values) in AT broth. Bacteria were cultured for 12 h and assayed for β-galactosidase activity. β-Galactosidase expression in the absence of autoinducers was less than 5 Miller units for both strains.