TABLE 5.

PCR amplification results from 18 different N. tabacum transgenes for pKP10 shuttle vector DNA, and from infecting bacterial strains, with various bacterium- or plant-related primers^a

DNA template	Amplified region	No. of plants containing each gene/ no. of plants tested
pKP10	nptII	18/18
pKP10	oriT(N)	18/18
pKP10	oriT ColE1	5/18
N. tabacum	Actin gene	18/18
E. coli	fliC	0/18
pKM101	repA	0/18

^aPrimers were designed so as to amplify gene segments specific for (i) vector pKP10 parts [the nptII kanamycin resistance gene, the tra(N) origin of transfer (oriT_N), and the ColE1 rep region], (ii) the N. tabacum chromosomal actin gene (positive control), and (iii) E. coli donor strain-specific genes (fliC chromosomal flagellar gene, plasmid pKM101 repA gene).