Figure 3.

Diagram of reporter gene constructs used for transfection studies and basal level expression of constructs in chicken heart primary cultures. (A) Map of cm2 genomic regions fused to the firefly luciferase gene. Solid line labeled +321 indicates exon/intron boundary. Thin dashed line indicates the most 3′ start site of transcription. Thick dashed line labeled 445 indicates PstI site. Construct names and approximate size of cm2 genomic fragments are shown at right. (B) Basal level of transcription for reporter gene constructs transfected into chicken heart cultures. Levels of luciferase expression normalized to β-gal expression (see Materials and Methods) are shown as fold increase over reporter gene vector (pGL3-Basic) containing no promoter. Results are averages ± SEM from 13 separate experiments each performed with six replicate cultures.