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. 2003 Nov;69(11):6888–6898. doi: 10.1128/AEM.69.11.6888-6898.2003

FIG. 2.

FIG. 2.

Southern hybridization with pBM400 and kanamycin gene probes to detect the integrated kanamycin gene. (A) Southern hybridization with a kanamycin gene DNA probe. Lane 1, E. coli V517 supercoiled standard; lane 2, QM B1551; lane 3, 54-kb plasmid pBM400::kan (from PV627); lane 4, QM B1551 containing pSM1 and a possible single crossover of pSM1 into pBM400; lane 5, pBM400::kan in the QM B1551 plasmid array following growth at 46°C; lane 6, PV361 chromosomal DNA. (B) Agarose gel electrophoresis of the same gel. (C) Southern hybridization with the pBM400-specific probe.