TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| B. megaterium | ||
| QM B1551 | Wild-type (7p+) B. megaterium | J. C. Vary, University of Illinois—Chicago |
| PV361 | Plasmidless (7p−) QM B1551 | 48 |
| QM B1551/pSM1 | Wild-type (7p+) B. megaterium plus pSM1 | This study |
| PV627 | PV361 containing only pBM400::kan | This study |
| E. coli | ||
| DH5α | F−Φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK−mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Gibco BRL |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ ΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ− rpsL nupG | Gibco BRL |
| V517 | Eight plasmids used as supercoiled standard | 30 |
| Plasmids | ||
| pJM103 | Apr in pUC19, Cmr from pC194 (integrative) (3.7 kb) | 39 |
| pHV33 | Apr Tcr from pBR322, Cmr from pC194 (7.3 kb) | 28 |
| pGEM3-Zf(+) | lacZ, Apr, F′ origin (3.7 kb) | Promega |
| pDG792 | pMTL23 containing Kmr cassette | 15 |
| pLTV1 | Tn917, Emr Tcr Cmr, lacZ, ori (E. coli), pE194ts ori (B. megaterium) | 7 |
| pKM31 | 2,152-bp XbaI fragment from pBM400 in pJM103 (5.8 kb) | This study |
| pKM58 | 1,045-bp XbaI-EcoRI pBM400 fragment in pJM103 (4.7 kb) | This study |
| pKM59 | 1.5-kb ClaI kanamycin gene (pDG792) in pKM58 (6.3 kb) | This study |
| pSM1 | 4-kb EcoRI oriE194ts (pLTV1) in pKM59 (10.3 kb) | This study |
| pSM3 | 8.7-kb EcoRI insert from pBM400 in pJM103 with ftsZ | This study |