TABLE 2.
Standard RT-PCR assay primers and conditions used in this study
| RT-PCR target | Primera | MgCl2 concn (mM) | Amplicon size (bp) |
|---|---|---|---|
| NV GI | |||
| RT | SRI-1 | ||
| PCR 1 | SRI-1/SRI-2 | 3.5 | 316 |
| PCR 2 | SRI-3/SRI-2 | 2.0 | 241 |
| NV GII | |||
| RT | SRII-1 | ||
| PCR 1 | SRII-1/SRII-2 | 3.5 | 514 |
| PCR 2 | SRII-3/SRII-2 | 1.5 | 203 |
| iSt-ENV synthetic RNA GI | |||
| GI | |||
| RT | SRI-1 | ||
| PCR 1 | SRI-1/SRI-2 | 3.5 | NDb |
| PCR 2 | SRI-3/SRI-2 | 2.0 | 221 |
| GII | |||
| RT | SRII-1 | ||
| PCR 1 | SRII-1/SRII-2 | 3.5 | ND |
| PCR 2 | SRII-3/SRII-2 | 1.5 | 184 |
a
Primer sequences were all previously described by Hafliger et al. (16).
b
ND, not determined.