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Quantitative RT-PCR assay primers and conditions used in this study
| RT-PCR target | Primer | Sequence | Amplicon size (bp) |
|---|---|---|---|
| NV GI, Valetta strain | |||
| RT | 9.4 Rev | GTCCTAACTGCAAATCAAAAAGCA | |
| PCR | 9.4 For | CCCAGGGCGAATTCACAA | 69 |
| PCR probe | TaqMan 9.4 | ATCCCCAAATAATACCCCCGGTGA | |
| NV GII, Hawaii strain | |||
| RT | 8.7 Rev | CTTCGCCCAGTAGGGACATC | |
| PCR | 8.7 For | CATGAAGACCCATCCGAAACA | 73 |
| PCR probe | TaqMan 8.7 | TGATACCACATTCCCAAAGACCCGTACAA |
