Figure 3.

NSE-LacZ-transgenic SVZ cells grafted into caudal SVZ of nontransgenic host migrate to the olfactory bulb where they differentiate into granular and periglomerular neurons. (A) Schematic drawing of horizontal hemisection indicating the position of the graft (asterisk) in the lateral wall of the lateral ventricle (LV) and the position of photomicrograph shown in B (rectangle). (B) Horizontal section of brain 17 (A-P −1.5, Table 1) showing graft site (arrow) relative to lateral ventricle (LV), corpus callosum (cc), and hippocampus (HP). (C) LacZ-positive granule neuron in the olfactory bulb 30 days after transplantation. Arrow indicates the Hoechst counterstained nucleus. (D) LacZ-positive periglomerular neuron (arrow) in the olfactory bulb 30 days after transplantation. The centers of glomeruli are indicated by stars. (E) Hoechst-stained horizontal olfactory bulb section from brain 17 with superimposed red dots indicating the distribution of LacZ-positive granular neurons and one LacZ-positive periglomerular neuron. OB, olfactory bulb; CB, cerebellum; gl, glomerular layer; epl, external plexiform layer; gcl, granule cell layer. (B, bar = 250 μm; C, bar = 50 μm; D, bar = 120 μm; E, bar = 400 μm.)