Darkfield (A, C, H, J, and
K) and fluorescence (D–G and
L) micrographs of the eye (A–G and
J) and nasal mucosa (H and
K–L) showing ppGAL mRNA (A, H, J, and
K) GAL-R1 mRNA (C) and GMAP-LI
(D–G and L) expression. B
and I show bisbenzimide counterstaining of the same
sections as in A and H, respectively. GAL
expression (arrows) is seen in the retina (r), with the highest level
at E17 (A, D, and F) and lower levels at
E15 (E) and E19 (G and J). No
distinct GAL-R1 mRNA signal is seen at E17 (C). A strong
ppGAL mRNA signal is seen in the dorsal aspects of the nasal mucosa at
E15 (H) and E21 (K). GMAP-LI (arrow) is seen in
olfactory epithelium (o) (L). c, cartilage. Small arrowheads
indicate nonspecific staining. (A–G, J, and
K, bar = 100 μm; H, I, and
L, bar = 200 μm.)