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Human telomeric DNA coimmunoprecipitated by an hTERT antibody after in vivo cross-linking in cells expressing nontagged HP1 proteins. ECR-293 cells overexpressing nontagged wild-type (HP1Hsα, HP1Hsβ, or HP1Hsγ) proteins were analyzed for hTERT interactions with telomeres, as described in the legend to Fig. 2. Note a decrease in the amount of telomeric DNA compared to that of the total genomic DNA in cells overexpressing nontagged HP1Hsα or HP1Hsβ.
