. 2003 Nov;23(22):8363–8376. doi: 10.1128/MCB.23.22.8363-8376.2003

TABLE 1.

Comparison of the frequencies of chromosome end-to-end associations at metaphase and bridges at anaphase in cells overexpressing various HP1 proteins^a

Cell type	Chromosome end associations/200 metaphases	Bridges/300 anaphases
Control	24	16
Control with empty vector	21	13
GFP-HP1^Hsα	94^b	49^b
GFP-HP1^Hsβ	88^b	43^b
GFP-HP1^Hsγ	32	15
HP1^Hsα	86^b	54^b
HP1^Hsβ	92^b	51^b
HP1^Hsγ	24	10
GFP-ΔHP1^Hsα	20	17
GFP-ΔHP1^Hsβ	22	11
GFP-ΔHP1^Hsγ	28	18

GFP fusion did not influence the function of HP1 proteins, as GFP-HP1 cells have an effect on chromosome aberrations similar to that seen in cells with overexpression of nontagged HP1.

The frequency is significantly different from that for the controls (ECR-293 cells) as assessed by chi-square analysis (P < 0.01).