Figure 3.

Eleven GCM-binding sites are present in the region upstream of the repo gene. (A) The GCM-binding sites. Positions of the GCM-binding sequence are indicated by solid ellipses (1–11). The consensus binding sequences are clustered in the proximal upstream 4 kb, whereas none were found between the −4 kb to −7-kb region. The initiation site and the orientation of the repo gene are indicated by a large arrow, according to Halter et al. (12). Xiong et al. (11) reported that one more exon is located at −4 kb in this figure, but we could not find such sequences within the −7-kb region. Thick bars and thin bars respectively show fragments that bound or did not bind to GCM in gel-shift assays. A and B indicate the probes used in the gel-shift assays shown in Figs. 1 and 2. E, EcoRI; S, SalI; and X, XhoI. (B) Alignment of the GCM-binding sequences in the upstream of repo. The numbers correspond to those in A. Nucleotides identical to the consensus sequence are highlighted. Three sequences that are in opposite orientation to the others are indicated by “r.”