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Kinetic models for RanGAP-catalyzed Ran-GTP hydrolysis and corresponding rate and equilibrium constants. (A) With RanBP1. (B) Without RanBP1. From the data presented here, it cannot be determined whether the rate limiting step kcat denotes the actual cleavage reaction or the Pi release step and whether or not Pi is released before or after RanGAP dissociation.
