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Localization of RanGAP (A and C) or chromatin (B and D) in either Saccharomyces cerevisiae wild-type (A and B) or rangapΔC362 (C and D) cells. RanGAP staining was performed using primary anti-RanGAP antibodies and secondary fluorescein isothiocyanate-labeled antibodies. Chromatin was stained with DAPI.
