FIG. 6.

ghrelin^−/− mice exhibit a normal orexigenic response to exogenous ghrelin. Cumulative food intake was measured in 12-week-old mice every 30 min for the duration of the experiment. Saline alone (100 μl) or saline containing 10 μg of ghrelin (Phoenix Pharmaceuticals, Inc.) was injected intraperitoneally into each mouse. Mice were first treated with saline and then 0.5 and 1.5 h later with saline containing ghrelin. For each gender group (n = 5), P < 0.05 (✽) in males and P < 0.001 in females (✽✽) (saline versus ghrelin).