TABLE 1.
Taqman primers and probes
| Target area (starting nt/ending nt) | Primer or probe (sequence) |
|---|---|
| HS2 5′ (−3447/−3367) | Forward (5′AGTCACTCTGTCTCACTGTGTCTTAGC) |
| Reverse (5′TTGTTGGAGGATACCCATTCTCTAT) | |
| Probe (5′6FAM-AGTTCCTTACAGCTTGCCCTGATGGGA-TAMRA) | |
| HS2 HindIII (−2735/−2664) | Forward (5′CTTAGTTCCTGTTACATTTCTGTGTGTCT) |
| Reverse (5′GGCCAGAACTGCTCATGCTT) | |
| Probe (5′6FAM-TAGTGACCTCCCATAGTC-TAMRA) | |
| HS2 NF-E2 (−2594/−2511) | Forward (5′GGCTCAAGCACAGCAATGC) |
| Reverse (5′CATCACTCTAGGCTGAGAACATCTG) | |
| Probe (5′6FAM-AGTCATGCTGAGGCTTAGGGTGTGTGC-TAMRA) | |
| HS2 XbaI (−2459/−2383) | Forward (5′AGGAGAAGCTGACCACCTGACT) |
| Reverse (5′CACATTCTGTCTCAGGCATCCA) | |
| Probe (5′6FAM-AAACTCCACCTCAAACGGCATCATAAAGAAA-TAMRA) | |
| HS2 AT-rich (−2299/−2209) | Forward (5′TGTTGTTATCAATTGCCATAGAATGA) |
| Reverse (5′CTGATTCTGCCGCTTCTAGGTATAG) | |
| Probe (5′6FAM-TTATCTTGCAGGTGGCC-TAMRA) | |
| HS2 3′ (−2124/−2051) | Forward (5′AAACAGGAAAAGAAAACAAACCTTGT) |
| Reverse (5′CACAGGCCTTTTGCCACCTA) | |
| Probe (5′6FAM-TGCCATTTTAAGGCACCCCTGGACAG-TAMRA) | |
| N6− (−1708/−1634) | Forward (5′GAATCCCCAAAGGCACTTGA) |
| Reverse (5′GGCCCTAGAGGTTCTTCGTCTT) | |
| Probe (5′6FAM-TGGGAGACAGGAGCCATACTGCTAAGTGA-TAMRA) | |
| N5− (−1204/−1107) | Forward (5′ATGGTGATGTAAGGGACTTTTATAGAATT) |
| Reverse (5′CTATCAATGTGACAGAAGTAATGATTGC) | |
| Probe (5′6FAM-CAATGCTGGAATTTGTGGAACTCTGCTTCTA-TAMRA) | |
| N4− (−725/−651) | Forward (5′GCCCCAGGCAATACTCACAA) |
| Reverse (5′CCAGGGAATCCTCTCTCTTTTCA) | |
| Probe (5′6FAM-AGCCAGTGTCCCCTGTGGTCATAGAGAA-TAMRA) | |
| N2− (−324/−240) | Forward (5′GATTTGCTCCTTTATATGAGGCTTTC) |
| Reverse (5′TTTTACCCTCTTCATCATCTTCCAA) | |
| Probe (5′6FAM-TGGAAAAGGAGAATGGGAGAGATGGATATCA-TAMRA) | |
| N1− (−161/−52) | Forward (5′CACAAACTTAGTGTCCATCCATCAC) |
| Reverse (5′CCCTGTTCTCCATGGTACTTAAAAG) | |
| Probe (5′6FAM-CTGACCCTCTCCGGACCTGACTCCAC-TAMRA) | |
| N1+ (18/86) | Forward (5′CAGCTGCAATCACTAGCAAGCT) |
| Reverse (5′GCAGCCTTCTCCTCAGCAGTA) | |
| Probe (5′6FAM-CAGGCCTGGCATCATGGTGCA-TAMRA) | |
| N2+ (300/371) | Forward (5′TTTTTTGACAGCTTTGGAAACCT) |
| Reverse (5′GCCATGGGCCTTGACCTT) | |
| Probe (5′6FAM-TCGTCTCCCTCTGCCATCCTGGG-TAMRA) | |
| N3+ (422/494) | Forward (5′CAAGCCCGCCTTTGCTAAG) |
| Reverse (5′CACCTTGAAGTTCTCAGGATCCA) | |
| Probe (5′6FAM-TGAGTGAGCTGCACTGTGACAAGCTGC-TAMRA) | |
| N4+ (940/1017) | Forward (5′ATTTCCCATCAGTATTGTGACCAA) |
| Reverse (5′AGAGTTCTCAGCGGGAGTTTAAAA) | |
| Probe (5′6FAM-TGAAGGCTTGTTTCCGAATTTGTTGGG-TAMRA) | |
| N5+ (137/1446) | Forward (5′CTACTCACTTTGGCAAGGAGTTCA) |
| Reverse (5′CGACAGCAGACACCAGCTTCT) | |
| Probe (5′6FAM-CCCTGAAGTGCAGGCTGCCTGG-TAMRA) |