Figure 4.

Localization of ZnT-3 mRNA, protein, and histochemically reactive zinc in the brain. Coronal sections of mouse brain were subjected to in situ hybridization with a ³³P-labeled probe complementary to mouse ZnT-3 mRNA (A and B), immunocytochemistry with affinity-purified antisera raised against the C-terminal tail of ZnT-3 (C and D), and Timm’s staining procedure for vesicular zinc (E and F). (B, D, and F, ×2.8; A, C, and E, ×0.7). In the hippocampus, ZnT-3 mRNA is present in the cell bodies of dentate granule cells, and ZnT-3 protein is abundant in the zinc-rich mossy fiber projections emanating from these neurons. A, amygdala (lateral nucleus); H, hippocampus; NCx, neocortex; PCx, piriform cortex; PV, paraventricular thalamic nucleus; ZI, zona inserta; GC, granule cell neurons of the dentate gyrus; hi, hilus; mf, mossy fibers. [Bars = 1000 μm (A) and 200 μm (B).]